STORE REQUISITION SLIP U.P. Pt. Deen Dayal Upadhyaya Pashu-Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura-281001 (UP)

Original Copy

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Letter No:	Date: 07-05-2026
College: College of Veterinary Science and AH	Department: Veterinary Medicine
Nature of Requirement: Recurring	Funding Source: University Grant

Note: Each Indent must pertain to only one category.

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Project/Scheme Title: Comparative evaluation of therapeutic efficacy and pro-inflammatory cytokine gene expression in dogs with flea allergy dermatitis treated with sarolaner and fipronil + (S)-methoprene

Sanctioned Amount (Enclosed Annexure ): ₹0.00

Purpose & Justification:
The chemical and kits are essential for the completion of approve MVSc thesis research project of my student (Dr Prerna Tomar), early procurement will be helpful in the timely completion of her MVSc degree.

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Details of Items:

S.N.	Item Description & Catalogue No (if applicable)	Specifications	Make/Brand(Only in case of RC)	Qty	Unit Cost (₹)	Approx Cost (₹)
1	RevertAid First Strand cDNA Synthesis Kit K1622	100 reaction	Thermofischer	1.00	34,000.00	34,000.00
2	Simport PCR tube	0.2 ml individual with cap TLS0851	biorad	1.00	3,844.00	3,844.00
3	Histopaque sigma 1077 10771	100 mL	Sigma	2.00	6,900.00	13,800.00
4	PCR Master Mix K0171	200 rxn	Thermofischer	1.00	13,000.00	13,000.00
5	SYBR Green Master Mix Power up A25741	5 ml	Thermofischer	1.00	21,000.00	21,000.00
6	DNA Ladder SM0373	50BP DNA Ladder	Thermofischer	1.00	13,690.00	13,690.00
7	Primers	300 bp	Eurosins	300.00	28.00	8,400.00
8	Trizol reagent 15596026	100 mL	Thermofischer	2.00	19,000.00	38,000.00
9	Microcentrifuge tube round bottom	500 units	TARSON	2.00	990.00	1,980.00
10	Microcentrifuge tube conical bottom	500 units	TARSON	2.00	990.00	1,980.00
11	Microtips 10µL AXT-10-B	500 units	AXIBIO	2.00	600.00	1,200.00
12	Microtips 100µL XT-200-B	500 units	AXIBIO	1.00	600.00	600.00
13	Microtips 1000µL AXT-1000-B	500 units	AXIBIO	2.00	375.00	750.00
14	Nitrile Gloves 33466-05		AXIBIO	4.00	900.00	3,600.00
15	Heparinized Vials	5 ml	LABRAM	1.00	900.00	900.00
16	EDTA Vials	5 ml	LABRAM	1.00	900.00	900.00
17	Plane Serum Vials	5 ml	LABRAM	1.00	900.00	900.00
18	Agarose powder 36601	100GM	SRL	1.00	5,600.00	5,600.00
19	RNaseZap AM9780	100 mL	Thermofischer	2.00	7,500.00	15,000.00
20	DEPC-Treated Water	100 mL	Thermofischer	1.00	5,600.00	5,600.00
21	Ethanol	500 mL	Sigma	1.00	2,500.00	2,500.00
22	PBS tablet	50 tablets (Pack)	Sigma	1.00	9,500.00	9,500.00

Specifications must be generic and non-proprietary (not brand specific).

	Sub Total:	₹196,744.00
	GST:	₹0.00
	Grand Total:	₹196,744.00

AMC Required: Yes

AMC Duration: N/A

Certification:
It is certified that the rates of the above items/services have been verified from different sources and found reasonable.( Enclosed rate reasonability Annexure )